Gel electrophoresis is able to separate DNA fragments strictly by relative size assuming

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The separation of DNA fragments during gel electrophoresis is based on their size and charge. The DNA fragments are negatively charged, and they move towards the positively charged electrode during electrophoresis, with smaller fragments migrating faster than larger fragments. The pH of the buffer is kept slightly alkaline (usually around pH 8.3) to ensure that the DNA fragments remain negatively charged and migrate appropriately based on size. Therefore, B is the best choice because it accurately describes the pH of the buffer used in gel electrophoresis, which is crucial for the separation of DNA fragments.

Gel Electrophoresis Is Able to Separate DNA Fragments Strictly by Relative