DNA testing by allele-specific oligonucleotide hybridization

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DNA testing by allele-specific oligonucleotide hybridization assumes that probes will bind to complementary sequences in the DNA. The method uses specific probes that are designed to hybridize only to the mutated sequence and not to the wild-type sequence. The presence or absence of hybridization indicates the genotype of the individual. This method can detect genetic abnormalities in heterozygotes, as long as the probes are designed appropriately. It does not require nonspecific oligonucleotide probes and does not rely on the presence of a restriction site. While it can be used to identify novel mutations, it is more commonly used to detect known mutations.

DNA Testing by Allele-Specific Oligonucleotide Hybridization