Which technique or tool listed below is best suited to determining the minimal genome of a free-living bacterium?
A) Metagenomics
B) Haplotype mapping
C) Hierarchical sequencing
D) Bacterial artificial chromosomes
E) Selective inactivation of genes

Question

Quizplus · Accepted Answer

Selective inactivation of genes allows for testing the functions of individual genes, which can help identify the ones that are essential for the bacterium's survival. By selectively inactivating genes and observing which ones are necessary for survival, the minimal genome can be determined.

Which Technique or Tool Listed Below Is Best Suited to Determining