Overlapping reads in high-throughput sequencing are possible because
A) the genetic code is redundant.
B) different individuals are sequenced.
C) the DNA is cut with different restriction enzymes.
D) computer programs and other bioinformatic tools are used.
E) synthetic oligonucleotides are used as primers.

Question

Quizplus · Accepted Answer

DOverlapping reads in high-throughput sequencing occur because the DNA is fragmented into many pieces, which can be sequenced from both ends or multiple points, leading to overlaps when the sequences are reconstructed. This process is facilitated by bioinformatic tools and computer programs designed to identify and align these overlapping sequences to reconstruct the original DNA sequence.

Overlapping Reads in High-Throughput Sequencing Are Possible Because