What is the major difference between using green fluorescent protein and using an antibiotic resistance gene as a reporter gene?
A) The green fluorescent protein can be used only when the host cell is a plant cell because it relies on the process of photosynthesis.
B) The antibiotic resistance gene can be used only when the host cell is an animal.
C) The green fluorescent protein can be used only in transfection, not in transformation.
D) With the green fluorescent protein, no cells are killed in the selection process.
E) Homologous recombination will work only with the green fluorescent protein.

Question

Quizplus · Accepted Answer

The major difference between using green fluorescent protein and using an antibiotic resistance gene as a reporter gene is that with the green fluorescent protein, no cells are killed in the selection process. In contrast, using an antibiotic resistance gene as a reporter gene requires killing of non-resistant cells using the antibiotic, whereas the green fluorescent protein allows for live selection of transformed cells based on fluorescence.

What Is the Major Difference Between Using Green Fluorescent Protein