A copy of the coding region of a gene is inserted downstream from a different, stronger promoter, and cells are transformed with the recombinant DNA.What is the most likely reason for the insertion?
A) To observe what happens when a gene is expressed at higher-than-usual levels
B) To observe what happens when a gene is expressed at lower-than-usual levels
C) To observe RNA expression levels
D) To generate a genomic library
E) To generate a cDNA library

Question

Quizplus · Accepted Answer

The insertion of the coding region downstream of a stronger promoter suggests that the purpose is to observe what happens when a gene is expressed at higher-than-usual levels. This could be done to study the effects of overexpression, or to produce a recombinant protein in higher yields.

A Copy of the Coding Region of a Gene Is