When would it be most appropriate to include a poly A-addition sequence in an expression vector?
A) When the transgene is of bacterial origin
B) When the transgene is of eukaryotic origin
C) When using a bacterial host cell
D) When using a eukaryotic host cell
E) Never

Question

Quizplus · Accepted Answer

The poly A-addition sequence is used to add a polyadenine tail to the mRNA transcript, which is necessary for stability and efficient translation in eukaryotic cells. Therefore, it is most appropriate to include a poly A-addition sequence in an expression vector when using a eukaryotic host cell.

When Would It Be Most Appropriate to Include a Poly