When would you add signal sequences to an expression vector?
A) When the transgene is of bacterial origin
B) When the transgene is of eukaryotic origin
C) When you want to express the gene only at certain times
D) When you want to express the gene only in certain tissues
E) When you want to direct the gene product to a specific location

Question

Quizplus · Accepted Answer

Signal sequences are added to expression vectors when the goal is to direct the gene product to a specific location within or outside the cell. This is particularly important when expressing eukaryotic genes, as they may have different localization requirements than bacterial genes. Adding a signal sequence can thus ensure correct targeting of the protein and increased efficiency of its function.

When Would You Add Signal Sequences to an Expression Vector