cDNA libraries
A) are the same as genomic libraries.
B) include DNA from noncoding sequences.
C) require DNA polymerase to generate.
D) are complementary DNA libraries made from mRNA.
E) contain all the genes of an organism.

Question

Quizplus · Accepted Answer

cDNA libraries are made from mRNA using the enzyme reverse transcriptase to generate complementary DNA (cDNA). This means that the library only contains DNA sequences corresponding to the expressed genes in the cell or tissue sample, rather than the entire genome (as would be the case for a genomic library). Therefore, cDNA libraries do not typically include noncoding sequences and do not require DNA polymerase to generate.

CDNA Libraries