A Researcher Is Interested in Determining What Nucleotide Substitutions in TTX-Resistant

A researcher is interested in determining what nucleotide substitutions in TTX-resistant puffer fish sodium channels are critical for resistance.To facilitate her research, she uses PCR-based in vitro evolution to generate altered versions of the puffer fish sodium channels.To increase the possibility of generating genes that are not resistant to TTX, the researcher should

A) use a DNA polymerase that has low fidelity.
B) not denature the DNA in between PCR cycles.
C) use DNA from other species of fish.
D) use DNA only from puffer fish that do not produce TTX.
E) limit the rounds of PCR to less than six.