The mass extinction at the end of the Ordovician period was probably caused by

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The mass extinction at the end of the Ordovician period was likely caused by massive glaciation on the southern continents and associated climate changes. This led to a significant drop in sea level and a decrease in oceanic circulation, which ultimately had a significant impact on marine life. Other factors such as volcanic activity and meteorite impacts may have played a role, but the evidence for these is less clear. The coming together of the continents to form Pangaea and changes in Earth's orbit occurred later, in the late Paleozoic and early Mesozoic eras.

The Mass Extinction at the End of the Ordovician Period