You are interested in studying bZIP1, a gene involved in nitrogen assimilation.The genomic copy of bZIP1 is full of introns, and to simplify your experimental design, you isolate the mRNA of this gene (which lacks introns).Which enzyme will be crucial in the next step of generating a cDNA version of bZIP1?
A) Oxygenase
B) RNA polymerase
C) Translocase
D) DNA polymerase
E) Reverse transcriptase

Question

Quizplus · Accepted Answer

To generate a cDNA version of bZIP1 from mRNA, reverse transcription is required. Reverse transcriptase is an enzyme that can convert RNA into complementary DNA (cDNA) using a primer to initiate synthesis. This cDNA can then be amplified by PCR to generate multiple copies for further analysis.

You Are Interested in Studying BZIP1, a Gene Involved in Nitrogen