You are interested in studying RPP13, a plant gene involved in disease resistance.The genomic copy of RPP13 is full of introns, and to simplify your experimental design, you isolate the mRNA of this gene (which lacks introns).Which enzyme will be crucial in the next step of generating a cDNA version of RPP13?

Question

Quizplus · Accepted Answer

Reverse transcriptase is needed to generate a complementary DNA (cDNA) version of the RPP13 mRNA. This enzyme is capable of synthesizing a single-stranded DNA using the mRNA as a template, which can subsequently be used to generate a double-stranded cDNA through the action of DNA polymerase. RNA polymerase is involved in the transcription of DNA to mRNA, and translocase and oxygenase are not relevant enzymes in this context.

You Are Interested in Studying RPP13, a Plant Gene Involved