Protoplasts Are Wall-Free Plant Cells That Can Be Produced in the Lab

Protoplasts are wall-free plant cells that can be produced in the lab by enzymatic digestion of the walls.Why are protoplasts more delicate than intact plant cells when they are placed in solutions of different concentrations?

A) The lack of a cell wall prevents osmosis.
B) The lack of a cell wall prevents the cells from shrinking.
C) The cell wall contains aquaporins required for water movement into the cell.
D) Cells that lack the cell wall cannot develop a pressure potential to counter the osmotic potential and could burst when placed in solutions having greater concentrations of solutes than are present in the cells.
E) Cells that lack the cell wall cannot develop a pressure potential to counter the osmotic potential and could burst when placed in solutions having lower concentrations of solutes than are present in the cells.