This still from Oskar Fischinger's film Circles is considered to be the first:

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The still from Oskar Fischinger's film Circles is considered to be the first experimental film. This film was made in 1933 and is a short animation featuring circles that grow, shrink, and move in various patterns. Fischinger was a pioneer in the use of experimental animation and Circles is considered to be one of his most influential works.

This Still from Oskar Fischinger's Film Circles Is Considered to Be