Which of the following is in the correct order for one cycle of polymerase chain reaction (PCR)?
A) Denature DNA; add fresh enzyme; anneal primers; add dNTPs; extend primers.
B) Add fresh enzyme; denature DNA; anneal primers; add dNTPs; extend primers.
C) Anneal primers; denature DNA; extend primers.
D) Extend primers; anneal primers; denature DNA.
E) Denature DNA; anneal primers; extend primers.

Question

Quizplus · Accepted Answer

The correct order for one cycle of PCR is to first denature the DNA (separate the two strands), then anneal primers (attach primers to each strand), and finally extend the primers (synthesize the new DNA strands).

Which of the Following Is in the Correct Order for One