Individual amino acids in protein hydrolysates may be effectively separated from each other by

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Chromatography on ion exchange resins is the best choice for separating individual amino acids from protein hydrolysates. This technique separates amino acids based on their charge and ionic properties, with different amino acids binding to the resin at different strengths and thus eluting at different times. Dialysis and ultracentrifugation are not effective for separating individual amino acids, while chromatography on Sephadex gels may separate larger peptides but is not as precise as ion exchange chromatography for individual amino acids.

Individual Amino Acids in Protein Hydrolysates May Be Effectively Separated