M17476PCR (Polymerase Chain Reaction) products are of discrete length because

Question

Quizplus · Accepted Answer

Polymerization proceeds to the ends of the DNA during PCR, resulting in products of discrete length. Taq polymerase is able to synthesize longer strands, so option A is incorrect. Denaturation of Taq polymerase after each cycle is not necessary for the generation of discrete PCR products, so option C is incorrect. Cutting the DNA with restriction enzymes prior to PCR would result in fragments of pre-determined lengths, but not in discrete PCR products, so option D is incorrect. A limiting amount of nucleoside triphosphate precursors would result in incomplete extension of PCR products, but not in discrete lengths, so option E is incorrect.

M17476PCR (Polymerase Chain Reaction) Products Are of Discrete Length Because