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Construction of a Plasmid Containing a Human Beta Globin CDNA

Question 73

Multiple Choice

Construction of a plasmid containing a human beta globin cDNA may involve all of the following EXCEPT


A) isolation of human beta globin mRNA.
B) use of poly dT as a primer for reverse transcriptase.
C) transcription with
D) homopolymer tailing of the cDNA and ligation of the tailed cDNA to the vector.
E) coli RNA polymerase.

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