For the citric acid cycle to proceed, it is necessary for

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In the citric acid cycle, an acetyl group from acetyl-CoA is combined with oxaloacetate to form citrate, the first compound in the cycle. Therefore, it is necessary for an acetyl group to bind to oxaloacetate for the cycle to proceed. Pyruvate and carbon dioxide do not directly bind to oxaloacetate in this process. Water is involved in other metabolic processes, but it is not directly involved in the citric acid cycle.

For the Citric Acid Cycle to Proceed, It Is Necessary