Suppose a DNA fragment from a human gene has been cut such that it has sticky ends. A DNA fragment from bacteria with sticky ends complementary to those of the human DNA is added to the mix. What would prevent the complementary ends of the human DNA from rejoining, and thus preventing the formation of a recombinant DNA molecule?

Question

Quizplus · Accepted Answer

The complementary sticky ends of the human and bacterial DNA can naturally anneal, and nothing would inherently prevent them from joining unless specific inhibitors or conditions are introduced.

Suppose a DNA Fragment from a Human Gene Has Been