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A plasmid has genes that confer resistance to both ampicillin and tetracycline. Foreign DNA is inserted at a restriction enzyme recognition site that lies within the ampicillin gene.
-After host cells have been given the opportunity to take up the plasmid, which of the following types of cells should be the most rare?

Question

Quizplus · Accepted Answer

Bacteria with the foreign DNA, but no plasmid should be the most rare because they would not have resistance to either ampicillin or tetracycline. The other types of cells would either have resistance to both antibiotics (C and D) or be able to survive on the selective media due to the presence of the plasmid (A and C). It is also possible that all types could appear with approximately the same frequency, but given the specific scenario described, B should be the most rare.

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