Which of the following statements is true?
A) A genomic library constructed from human brain tissue will likely be different than a gene library constructed from human pancreatic tissue.
B) A cDNA library constructed from human brain tissue will likely be different than a cDNA library constructed from human pancreatic tissue.
C) A cDNA library would be very useful in examining DNA that does not code for mRNA.
D) Messenger RNAs have a long life span in the cytoplasm.
E) All of the above

Question

Quizplus · Accepted Answer

cDNA libraries are constructed from complementary DNA produced by reverse transcription of mRNA. Therefore, they only contain expressed genes found in the tissue at the time of sampling. A genomic library, on the other hand, contains all DNA sequences in the genome, including non-coding regions and introns. Therefore, choice A is incorrect. Choice C is also incorrect as cDNA only represents expressed genes. Choice D is false as mRNA has a short lifespan in the cytoplasm.

Which of the Following Statements Is True