Cloning a gene may involve
A) restriction endonucleases and ligase.
B) plasmids and bacteriophage $\lambda$.
C) transformation or transfection.
D) selectable markers and/or reporter genes.
E) All of the above

Question

Quizplus · Accepted Answer

Cloning a gene typically involves the use of multiple techniques, such as restriction endonucleases and ligase to cut and paste DNA fragments together, plasmids and bacteriophage for inserting the gene into a host cell, transformation or transfection to introduce the gene into host cells, and selectable markers and/or reporter genes to identify which host cells have successfully integrated the gene. Therefore, all of the above (A, B, C, D) are involved in gene cloning.

Cloning a Gene May Involve A) Restriction Endonucleases and Ligase λ\lambdaλ

Cloning a Gene May Involve
A) Restriction Endonucleases and Ligase $\lambda$