A 10 base-pair single-stranded DNA fragment was cut by three different restriction enzymes to produce the following fragments:
(1) AC, TAC, and GGATG
(2)TA, CGG, and ATGAC
(3) GAC, GAT, and TACG.
The nucleotide sequence of the ten base fragment is

Question

A 10 base-pair single-stranded DNA fragment was cut by three different restriction enzymes to produce the following fragments: 
(1) AC, TAC, and GGATG
(2)TA, CGG, and ATGAC
(3) GAC, GAT, and TACG.
The nucleotide sequence of the ten base fragment is

Quizplus · Accepted Answer

By comparing the fragments produced by each restriction enzyme, we can determine the location of the restriction sites. The first enzyme cuts after A and before C, producing AC and TAC. The second enzyme cuts after T and before G, producing TA and CGG. The third enzyme cuts after G and before T, producing GAC and TACG. The overlapping fragments in all three sets are TAC and GAT, indicating that these two nucleotides must be next to each other in the original sequence. Therefore, the possible sequence is TACGGATGAC.

A 10 Base-Pair Single-Stranded DNA Fragment Was Cut by Three