Shotgun DNA Sequencing Differs from Hierarchical DNA Sequencing in That

Shotgun DNA sequencing differs from hierarchical DNA sequencing in that

A) fragments with overlapping sequences are not needed for shotgun sequencing.
B) specific markers are required for hierarchical DNA sequencing.
C) shotgun DNA sequencing does not require amplification of the DNA.
D) multiple cutting of the DNA is not needed for shotgun DNA sequencing.
E) genomic libraries are used only in shotgun sequencing.