Which of the following techniques relies on the same principle as RFLP?
A) Amniocentesis
B) Screening by allele-specific oligonucleotide hybridization
C) Screening by allele-specific cleavage
D) Guthrie's test for PKU
E) Preimplantation screening

Question

Quizplus · Accepted Answer

Screening by allele-specific cleavage, also known as PCR-RFLP, relies on the same principle as RFLP (restriction fragment length polymorphism). In this technique, PCR is used to amplify a specific region of DNA, which is then digested with a restriction enzyme that recognizes a specific polymorphic site. The presence or absence of the polymorphism can be detected by analyzing the size of the resulting fragments on an agarose gel.

Which of the Following Techniques Relies on the Same Principle