Which of the following statements is true regarding allele-specific oligonucleotide hybridization?
A) It uses oligonucleotide probes.
B) A minimum of a dozen bases is required.
C) The probe may be radioactive or fluorescent so hybridization can be easily detected.
D) It can reveal whether individuals are heterozygous.
E) All of the above

Question

Quizplus · Accepted Answer

Allele-specific oligonucleotide hybridization uses oligonucleotide probes, typically with a minimum length of a dozen bases, and the probes may be labeled with either a radioactive or fluorescent tag to enable easy detection of hybridization. The technique can reveal whether individuals are heterozygous, making statement D also true. Therefore, all of the statements are true.

Which of the Following Statements Is True Regarding Allele-Specific Oligonucleotide