DNA testing by allele-specific hybridization

Question

Quizplus · Accepted Answer

DNA testing by allele-specific hybridization requires PCR to amplify the gene in question. This amplification step allows for the detection of even small amounts of the target DNA sequence, making the technique highly sensitive. A non-specific oligonucleotide probe is not required since the probe is designed to specifically hybridize with the target allele. The technique can detect genetic abnormalities in heterozygotes as long as the mutant allele is present in the sample. The mutation does not need to be in a restriction site, as is required for RFLP analysis. DNA testing by allele-specific hybridization is also more precise than RFLP analysis since it detects specific mutations rather than relying on variations in restriction sites.

DNA Testing by Allele-Specific Hybridization