When double stranded DNA is heated to about 90ºC it denatures. Denaturation
A) involves hydrolysis of the DNA.
B) destroys the double helical structure irreversibly.
C) separates the two strands.
D) causes new hydrogen bonds to form.
E) breaks covalent bonds.

Question

Quizplus · Accepted Answer

Denaturation involves the separation of the two strands of DNA, and is not associated with the hydrolysis or cleavage of any chemical bonds within the molecule. While denaturation can be reversible under certain conditions, it does initially disrupt the double helical structure of the DNA. Additionally, denaturation does not involve the formation of new hydrogen bonds or the breaking of any covalent bonds.

When Double Stranded DNA Is Heated to About 90ºC It