When the pentapeptide below is heated first with 2,4-dinitrofluorobenzene (and base)and then subjected to acidic hydrolysis,which amino acid will bear the dinitrophenyl group? Leu·Val·Gly·Phe·Ile

Question

Quizplus · Accepted Answer

The 2,4-dinitrofluorobenzene (Sanger's reagent) reacts with the N-terminal amino acid of a peptide. In this pentapeptide, Leucine is the N-terminal amino acid, so it will bear the dinitrophenyl group after the reaction and hydrolysis.

When the Pentapeptide Below Is Heated First with 2,4-Dinitrofluorobenzene (And