An enzyme that displays negative cooperativity has ____ activity when the substrate is present at concentrations ____ than the value of K0.5 when compared with enzymes that display no cooperativity.
A) increased; greater
B) increased; less
C) decreased; greater
D) decreased; less
E) both b and c are correct

Question

Quizplus · Accepted Answer

Negative cooperativity means that the binding of the first substrate molecule decreases the enzyme's affinity for subsequent substrate molecules. Therefore, when the substrate concentration is less than K0.5, the activity is increased because the enzyme is less likely to bind to additional substrate molecules, making it more active per bound substrate. Conversely, when the substrate concentration is greater than K0.5, the activity is decreased because the enzyme's affinity for the substrate is lower, making it less efficient at catalyzing the reaction.

An Enzyme That Displays Negative Cooperativity Has ____ Activity When