In the hydrolysis of p-nitrophenylacetate by chymotrypsin all of the following are correct EXCEPT:
A) the first product is acetic acid.
B) an acetyl-enzyme intermediate forms during the mechanism.
C) attack of a water molecule on the acyl-enzyme intermediate yields the second product.
D) the active site contains a serine residue, an unprotonated histidine, and an unprotonated aspartate residue at pH 7.0 prior to binding the substrate.
E) none of the above.
Correct Answer:
Verified
Q2: In the catalytic triad common to many
Q3: All are true for low-barrier hydrogen bonds
Q4: All are true for the enzyme-transition state
Q5: Most covalent catalysis is carried out by
Q6: The initial bond formation in the covalent
Q7: Because the pKa is near 7, _
Q8: Which of the following statements regarding enzymes
Q9: Metal ion catalysis include all EXCEPT:
A) increased
Q10: The transition state has an estimated life-time
Q11: The good transition state analog is one
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