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Each Cycle of Amplification in PCR Involves All of the Steps

Question 26

Multiple Choice

Each cycle of amplification in PCR involves all of the steps EXCEPT:


A) the addition of fresh dNTPs to the reaction mixture.
B) annealing of oligodeoxyribonucleotide primers to DNA.
C) thermal denaturation of the target duplex DNA.
D) reaction with DNA polymerase at approximately 70°C.
E) none of the above.

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