Which of the following statements correctly describes the process of DNA sequencing by chain termination?
A) the enzyme DNA polymerase is used to form a primer that binds to the template DNA
B) in order to effectively sequence the DNA, the dideoxynucleotides must be present at a higher concentration than the deoxynucleotides
C) the use of 4 unique fluorescent labels attached to the dideoxynucleotides allows for a single reaction to be run
D) the dideoxynucleotides lack a 5'-OH and therefore can not serve as acceptors in nucleotide addition
E) both B and C are correct

Question

Quizplus · Accepted Answer

The process of DNA sequencing by chain termination, also known as Sanger sequencing, utilizes 4 unique fluorescent labels attached to the dideoxynucleotides (ddNTPs). This allows for all four ddNTPs to be mixed in a single reaction, where each type of ddNTP terminates the DNA strand extension at a different point. The resulting fragments can then be separated by size using capillary electrophoresis, and the sequence can be read based on the color of the fluorescent labels.

Which of the Following Statements Correctly Describes the Process of DNA