After treating a protein with trypsin, which of the following techniques could be used to determine its identity by peptide mass fingerprinting?
A) NMR
B) MALDI-TOF mass spectrometer
C) HPLC
D) gel electrophoresis
E) none of the above

Question

Quizplus · Accepted Answer

MALDI-TOF mass spectrometry is the most commonly used technique for peptide mass fingerprinting. It can identify the peptides based on their masses, allowing for the identification of the original protein. NMR is not commonly used for this application, HPLC is not specific for protein identification, and gel electrophoresis is used for separating and visualizing proteins but not for identifying them.

After Treating a Protein with Trypsin, Which of the Following