The advantage of treating separate samples of a protein with two or more enzymes when sequencing a protein is that the products are:
A) more homogeneous.
B) sequenceable without further chromatography.
C) fragments with the same N- and C-terminal amino acids.
D) fragments with sequence overlaps.
E) all are true.

Question

Quizplus · Accepted Answer

Treating separate samples of a protein with two or more enzymes results in fragments with sequence overlaps, which can help assemble the full protein sequence. This is especially useful when dealing with larger proteins where sequencing the full protein at once may be difficult.

The Advantage of Treating Separate Samples of a Protein with Two