Which of the following is the first step in chromatin immunoprecipitation?
A) Extraction of chromatin from cells
B) Cross-linking with formaldehyde
C) Shearing by sonication
D) Addition of antibodies to recognize proteins that bind to DNA
E) PCR to amplify the target sites

Question

Quizplus · Accepted Answer

The first step in chromatin immunoprecipitation (ChIP) is cross-linking with formaldehyde, which covalently links proteins to DNA. This is done to preserve the interactions between DNA and its associated proteins in vivo.

Which of the Following Is the First Step in Chromatin