Evidence supporting E.coli DNA polymerase III having the major role in nucleotide incorporation during replication includes:

Question

Quizplus · Accepted Answer

All of the given options provide evidence supporting the major role of E.coli DNA polymerase III in nucleotide incorporation during replication.

Option A states that DNA polymerase III has a high Vmax, indicating that it has a high rate of nucleotide incorporation.

Option B indicates that there are only ten or fewer molecules of enzyme per cell, suggesting that the few DNA polymerase III molecules must be highly efficient and specialized for their function.

Option C further supports the importance of DNA polymerase III by demonstrating that replication is blocked in temperature-sensitive mutants lacking functional DNA polymerase III.

Option D demonstrates that a single point mutation in DNA polymerase III can impair its function and inhibit replication.

Therefore, all of the given options together provide evidence that E.coli DNA polymerase III plays a major role in nucleotide incorporation during replication.

Evidence Supporting E