The fluorescence imaging method in which an excitation laser is focused into a thin,flat sheet that illuminates the sample along a plane perpendicular to the angle of observation is known as:
A)Fluorescence recovery after photobleaching
B)Laser scanning confocal microscopy
C)Super-resolution microscopy
D)Light sheet microscopy
E)Fluorescence resonance energy transfer

Question

Quizplus · Accepted Answer

The fluorescence imaging method described in the question is known as light sheet microscopy, also referred to as selective plane illumination microscopy (SPIM). This technique is distinct from other forms of fluorescence microscopy such as confocal microscopy, super-resolution microscopy, and fluorescence resonance energy transfer. Fluorescence recovery after photobleaching (FRAP) is a technique used to study the mobility and dynamics of proteins within living cells.

The Fluorescence Imaging Method in Which an Excitation Laser Is