_________ are established with cells that have been frozen in liquid nitrogen,thawed and then placed in a waiting culture medium.They are usually previously cultured cells that were frozen for storage.
A)Primary cultures
B)Secondary cultures
C)Tertiary cultures
D)Supernatant cultures
E)Penultimate cultures

Question

Quizplus · Accepted Answer

Secondary cultures are established with cells that have been thawed from frozen storage and placed in a waiting culture medium. They are considered to be the second generation of cells, as they were previously cultured and then frozen for storage. Primary cultures are established directly from tissues, while tertiary cultures and penultimate cultures are not commonly used terms in cell culture. Supernatant cultures refer to the liquid portion of a cell culture after the cells have been removed, so it is not an appropriate choice.

_________ Are Established with Cells That Have Been Frozen in Liquid