Which of the following is most likely to elute first from a gel filtration column if each of them is essentially globular?
A)a 250 kilodalton protein
B)a 120 kilodalton protein
C)a chromate ion
D)a 12 kilodalton protein
E)an 80 kilodalton protein

Question

Quizplus · Accepted Answer

In gel filtration chromatography, larger molecules elute first because they are excluded from the pores of the gel and travel a shorter path through the column. The 250 kilodalton protein is the largest and will elute first.

Which of the Following Is Most Likely to Elute First