In the molecular beacon procedure for quantifying a DNA or RNA template,what role does the quencher play?
A)It enhances fluorochrome fluorescence.
B)It suppresses fluorochrome fluorescence.
C)It inhibits exonuclease activity.
D)It enhances exonuclease activity.
E)It enhances endonuclease activity.

Question

Quizplus · Accepted Answer

The quencher plays a crucial role in the molecular beacon procedure by suppressing the fluorescence of the fluorochrome attached to the probe when it is not hybridized to the target template. This quenching ability ensures that the probe remains dark in the absence of the target template, thus preventing any false-positive signals. When the molecular beacon probe hybridizes to the target template, the quencher is separated from the fluorochrome, allowing fluorescence to be detected.

In the Molecular Beacon Procedure for Quantifying a DNA or RNA