If the template in the cycle sequencing method is a PCR product,what is generally used as a primer?
A)both PCR primers
B)randomly generated primers
C)one,but only one,of the PCR primers
D)any primer but the PCR primers
E)poly(A)oligonucleotides

Question

Quizplus · Accepted Answer

When using a PCR product as the template in the cycle sequencing method, it is common to use only one of the PCR primers as the sequencing primer. This is because using both PCR primers could result in overlapping peaks in the sequencing data, making it difficult to interpret the sequence. Randomly generated primers or poly(A) oligonucleotides are not typically used as sequencing primers in this scenario. While any primer can technically be used, using a primer other than one of the PCR primers could result in a lower yield of sequencing data.

If the Template in the Cycle Sequencing Method Is a PCR