You isolate superoxide dismutase from two cell culture lines.One of the lines (SOD1)has a level of SOD activity similar to that found in liver,the tissue from which the cell line was originally obtained.The other cell line (SOD10)has elevated SOD activity.The enzyme in SOD10 is extremely efficient at converting the superoxide free radical to hydrogen peroxide.In a routine check of other critical enzyme activities,catalase was found to have activity levels that were severely depressed in SOD10,while they appeared normal in SOD1.Observations of SOD10 reveal that this cell line cannot be maintained as easily as SOD1.SOD10 cells appear to die at an accelerated rate.What,if anything,can you conclude from these data?

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The Answer of You isolate superoxide dismutase from two cell culture lines.One of the lines (SOD1)has a level of SOD activity similar to that found in liver,the tissue from which the cell line was originally obtained.The other cell line (SOD10)has elevated SOD activity.The enzyme in SOD10 is extremely efficient at converting the superoxide free radical to hydrogen peroxide.In a routine check of other critical enzyme activities,catalase was found to have activity levels that were severely depressed in SOD10,while they appeared normal in SOD1.Observations of SOD10 reveal that this cell line cannot be maintained as easily as SOD1.SOD10 cells appear to die at an accelerated rate.What,if anything,can you conclude from these data?

You Isolate Superoxide Dismutase from Two Cell Culture Lines