What step must first be carried out before one can amplify RNA templates?
A)Denaturation must be carried out first.
B)They must first be converted to complementary DNAs using DNA polymerase.
C)They must first be converted to complementary RNAs using RNA polymerase.
D)They must first be converted to complementary DNAs using reverse transcriptase.
E)They must first be converted to complementary DNAs using Taq polymerase.

Question

Quizplus · Accepted Answer

The step that must first be carried out before amplifying RNA templates is the conversion of RNA templates to complementary DNAs using reverse transcriptase. This process is called reverse transcription, and it allows for the RNA template to be converted into a complementary DNA template that can be used for PCR amplification.

What Step Must First Be Carried Out Before One Can