In an attempt to monitor the movement of individual kinesin molecules labeled with GFP along microtubules labeled with a red fluorescent dye using an in vitro motility assay,what specialized type of laser-based fluorescence microscopy would be most likely to be employed?
A)FRAP
B)TIRF
C)total internal reflection microscopy
D)atomic force microscopy
E)TIRF and total internal reflection microscopy

Question

Quizplus · Accepted Answer

TIRF (Total Internal Reflection Fluorescence) microscopy is specifically designed to observe events at or near the cell surface, such as the movement of molecules along microtubules, by exciting fluorophores in a very thin region. Total internal reflection microscopy is another name for TIRF, so both terms refer to the same technique.

In an Attempt to Monitor the Movement of Individual Kinesin