TIRF Works by _________________

TIRF works by _________________.

A) chemically dissecting microtubules
B) focusing on a very thin plane just above the surface on which microtubules are lying
C) by preventing light rays emanating from individual motor proteins being monitored from being obscured by light rays from other areas
D) focusing on a very thin plane just above the surface on which microtubules are lying and by preventing light rays emanating from individual motor proteins being monitored from being obscured by light rays from other areas
E) denaturing the tubulin subunits of microtubules