A control cell that is synthesizing a GFP-labeled version of mannosidase II has fluorescence localized in the numerous Golgi complexes of the cell.Normally,this enzyme is synthesized in the endoplasmic reticulum and moves via transport vesicles to the Golgi complex,where it takes up residence.If an experimental cell contains an siRNA that leads to the fluorescence being restricted to the endoplasmic reticulum,with what would the siRNA be likely to interfere?
A)an mRNA that codes for a protein involved in the transport of the enzyme from the ER to the Golgi complex
B)an rRNA that synthesizes the enzyme
C)the synthesis of mannosidase II from its mRNA
D)an mRNA that codes for a protein involved in the transport of the enzyme from the Golgi complex to the ER
E)an mRNA that codes for the enzyme

Question

Quizplus · Accepted Answer

The siRNA is likely interfering with the mRNA that codes for a protein involved in the transport of the enzyme from the ER to the Golgi complex. Without this transport protein, the mannosidase II enzyme cannot reach its destination in the Golgi complex and instead accumulates in the ER, resulting in restricted fluorescence. The other answer choices are not relevant to the observed result.

A Control Cell That Is Synthesizing a GFP-Labeled Version of Mannosidase