About What Time Does PI(4,5)P₂ Disappear from a Site of Endocytosis

About what time does PI(4,5) P₂ disappear from a site of endocytosis?

A) about the time the coated pit forms
B) about the time the coated vesicle is pinched away from the plasma membrane
C) when the ligand binds to the receptors
D) well after the vesicle has pinched away from the plasma membrane
E) as the coated pit rounds into a larger coated pit